| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 11 | Sinorhizobium fredii | AACGCTCGACATCGCCTT | GGAAGGAGAGCAGCGTGAA | 59.74 | 59.71 | 161 | 98 |
100.00%
|
100.00%
|
| Primer 12 | Sinorhizobium fredii | AACGCTCGACATCGCCTT | TTCGAGAGACCGAGCCACT | 59.74 | 60.30 | 228 | 98 |
100.00%
|
100.00%
|
| Primer 13 | Sinorhizobium fredii | TCATGCTGGCACGGTTCA | TCATCACGCCAACGAGCA | 59.57 | 59.66 | 234 | 96 |
100.00%
|
100.00%
|
| Primer 14 | Sinorhizobium fredii | AGAGTTCGTCGGTGCTACG | ATCGCCTGTTGGTCGTGT | 59.50 | 59.26 | 222 | 96 |
100.00%
|
100.00%
|
| Primer 15 | Sinorhizobium fredii | AGGACAATTGGCTGGAGGC | AGCAGCTTTTCCAGGCGT | 60.00 | 59.89 | 280 | 94 |
100.00%
|
100.00%
|
| Primer 16 | Sinorhizobium fredii | ACGGAGGACAATTGGCTGG | AGCAGCTTTTCCAGGCGT | 60.00 | 59.89 | 284 | 94 |
100.00%
|
100.00%
|
| Primer 17 | Sinorhizobium fredii | TGATCGAGCGGAAATGGGG | AGCAGCTTTTCCAGGCGT | 59.85 | 59.89 | 205 | 94 |
100.00%
|
100.00%
|
| Primer 18 | Sinorhizobium fredii | ATTGGCTGGAGGCGATCAG | AGCAGCTTTTCCAGGCGT | 59.85 | 59.89 | 274 | 94 |
100.00%
|
100.00%
|
| Primer 19 | Sinorhizobium fredii | ACTGCCTCGACATGCACA | CGGAAACTGATCGCCATGC | 59.26 | 59.65 | 277 | 92 |
100.00%
|
100.00%
|
| Primer 20 | Sinorhizobium fredii | ACTGCCTCGACATGCACA | GCGGAAACTGATCGCCATG | 59.26 | 59.65 | 278 | 92 |
100.00%
|
100.00%
|